We are proud to announce the acceptance and presentation of “Oxygen Content – Uncontrolled and Overlooked Parameter Associated with Stored Red Cell Concentrate: Unexpectedly Wide Distribution” abstract and poster at the recent International Society of Blood Transfusion (ISBT) meeting which took place recently in Copenhagen.

Oxygen is the main substrate for oxidative reactions and resulting oxidative damages are considered as one of the major causative factors of red cell storage lesion development. This study was conducted to understand the hemoglobin oxygen saturation levels (%SO2), surrogate for total O2 concentration, among donated units of leukoreduced RCC at Rhode Island Blood Center (RIBC), Providence, RI.  977 units of leukoreduced RCC were examined for %SO2 non-invasively employing Resonance Raman device within 8 hours of blood collection from January 9-13, 2017, representing 78% of collected units during this period.

%SO2 of venous blood is generally assumed to be around 70-80% as measured from a central venous line.  However, a recent investigation of %SO2 levels in freshly procured red cell concentrates (RCC) revealed unexpectedly wide – distribution of %SO2 (mean 45.9±17.5%) and at the same time, demonstrated negative consequences of high %SO2 levels in red blood cell (RBC) quality during hypothermic storage 1-6°C [Yoshida T, Blair A, D’Alessandro A, et al. Enhancing uniformity and overall quality of red cell concentrate with anaerobic storage. Blood Transfus 2017;15:172-81}.

The data collected in this recent study at RIBC supports our previous findings in the surprisingly wide distribution of starting %SO2 levels of freshly prepared RCC.  Considering recent reports showing profound effects of oxygen levels on RBC metabolism, coupled with the negative impacts of high oxygen saturation on stored RCC quality suggests that oxygen levels are an important and underappreciated source of unit to unit variability in RBC quality.

Due to our recent findings, we continue to work with interested blood centers to examine the %SO2 in their donated units of red cells.

Please click here to read the abstract.